Fig. 5.

The phosphoproteome analysis of MV4–11 perturbed with Midostaurin. A Barplot represents the number of quantified PS in every replicate before imputation for MV4–11 exposed to control (CTRL, red) or 50 nM Midostaurin (DRG, green) conditions. B, C Inhibition of FLT3 downstream reference, pSTAT5A/B Y694/Y699 as shown by WB and MS. The original western blots can be found in the additional file 2. D KAEA waterfall plot showing overactive (red) and underactive kinases (blue) after exposure of MV4–11 to Midostaurin. E STRING kinase-signaling network of significantly positive (red) and negative (blue) enriched kinases. The magenta edged kinases with asterisks (*) highlight experimentally validated targets of Midostaurin. TS: tumor suppressor