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. 2021 Jul 3;22(13):7195. doi: 10.3390/ijms22137195

Figure 1.

Figure 1

Establishment of doxycycline (Dox)-inducible Amelx-expressing mouse iPSC (Amelx-iPSC) line. (A) Generation of Dox-inducible Amelx-expressing piggyBac vector (PB-Amelx). (B–D) Inducible Amelx expression in Amelx-iPSCs after 24 h of culture with different concentrations of Dox (0–2 μg/mL) was examined by semi-quantitative RT-PCR (B) and Western blot (C) along with mCherry expression (D). (E,F) Amelx-iPSCs (A-iPSCs) showed pluripotency comparable to the original iPSCs, as determined by pluripotency marker gene expression (semi-quantitative RT-PCR) (E), ALP staining, and immunofluorescence for SSEA-1 and Nanog (F). Scale bars: 100 μm.