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. 2021 Jul 3;22(13):7195. doi: 10.3390/ijms22137195

Figure 4.

Figure 4

Gene expression profile of ameloblast differentiation markers during the stepwise induction of Amelx-iPSCs. (A) Diagram of an optimized protocol for stepwise ameloblast induction from mouse iPSCs. (B) Gene expression of stemness (Oct4) and ameloblast differentiation (dental epithelial cells (DECs): Krt14, p75, and tuftelin; and ameloblasts: Amelx and Ambn)) markers at each stage determined by real-time RT-PCR during stepwise induction of ameloblast lineage from Amelx-iPSCs. Different letters among groups (e.g., a, b) indicated significant differences (p < 0.05; one-way ANOVA and Tukey’s test; n = 3). (C) Summary of gene expression profile of ameloblast differentiation markers during the stepwise induction of mouse iPSCs.