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. 2021 Jun 25;13(13):3182. doi: 10.3390/cancers13133182

Figure 5.

Figure 5

p73-isoforms are dispensable for mESC self-renewal but required for appropriate expression of G1 phase Cyclins/Cdk complexes. (A) Short-term and long-term growth curves of the indicated cell lines. Three independent experiments, with three replicates per condition, were performed. (B) Representative cell cycle profiles of the control and knockout cell lines. The quantification of the percentage of cells in each cell cycle phase using the Watson improved model showed no differences between genotypes. Bar graphs represent the mean ± SEM from three independent experiments. (C) Differential gene expression analysis of cell cycle-related genes (RNA-seq data) in E14-TA- and E14-DN-p73KO cells compared to E14-WT controls at day 0. (D,E) Analysis of Ccnd2 and Cdk6 upregulation by qRT-PCR analysis in E14-TAp73KO cells, respectively. * p-value < 0.05, ** p-value < 0.01, *** p-value < 0.001.