Table 5.
The list of primers used in the study.
| Gene | Primer Sequence (5′→3′) | Application |
|---|---|---|
| AsPR1a, b | ATGGAACACGACACTGGCAG GCATACTGACCAGAGTAACTGG |
Gene expression analysis (qRT-PCR) |
| AsPR1c | GGCGGTCCTTATGGTGAAA GCCAGGGTCACATGTGTTA |
|
| AsPR1d | GGCGGTCCTTATGGTGAAA CCAGGGTCACATGTGTTGCT |
|
| AsPR1f, g | CGATCACCACCGCAGTTCA GCGTAGTTCTGTGCGTAATCAG |
|
| AsPR1i | TATGGGGAGAACCTATTCGC AATCTTRACCGACTTAGCCCA |
|
| AsPR1k | GTGTCCGAGAAGCGGTACTAT AGCCGCCAGTGTTGCACC |
|
| AsPR1p | GTCGCAAAATACGCGCAAAGTT GTACTTCACGACATCGGCATC |
|
| AsPR2a | GCTAGAAACCATATCGTTGCCT GCATACCGTAGCATACTCCGA |
|
| AsPR2b | GGTCGCATTTCTCCTAGGCAT GCGTCGCCTGCTGATGGAA |
|
| AsPR2c | GGCCCATTGTCCAGTTCTTG AGGCGCCGTGAATAATGCGTA |
|
| AsPR4a | ATGCCGGCATGTCCCTCG GTCTATGATCCTCACCGTCGTT |
|
| AsPR4b | ATGCCGGCATGTCCCTCG GGTCTATGATCCTCACCGTCAA |
|
| AsPR5a | CATCCGGACACGGCAGCT TCCATGTACTGCTTCAGAGCG |
|
| AsPR5c | GCAAGCAGCTCAACTCAGGA GCCGGTCTGACATCTTCCA |
|
| AsPR1c | ATGGGATCAATCAGTAGTTATA AACGACTGAGTACTCTCAGT |
Gene amplification |
| AsPR1d | ATGGGATCGACCAGTACTTG TAACGTCGTAGTTGTAACGAC |
|
| AsPR1f, g | ATGAAAACGTCATTTCTCTTC ATAAATAGCAGTACACACATAA |
|
| AsPR1k | GCTCAAATTACAATGAAAACGTT CTGTCTGTTTCAGCATGCA |
|
| AsPR2a | TGTGCACCATCGAATTACCTTC CTCTGTCTCCCTTAATAGTAC |
|
| AsPR2b | AAATGCAAGCAAGGAAGCTTG CCCTGGTACATTCATAGTTAAC |
|
| AsPR2c | TTGAAATGGTCATGCATGCCT AAACAGGGCACACATGCAAG |
|
| AsPR5a | ATGTCGACCCAAATTACAGTC ACTCAATCCAAGAAYACAGTTC |
|
| AsPR5c | ATGTCGACCCAAATTACAGTC CTGCAAACTAATTATTCGGTGA |