Figure 5.

Removing C1orf132 p2 genomic region using three different guide RNAs. (A) The genomic locations targeted with guide RNAs are depicted as guides 1–3 on putative p2 region (highlighted by purple color), (B) following transfection of different pairs of guide RNAs into MCF12A cells, using gRNAs 1 and 3 led to the generation of a sharp PCR product of 438 bps caused by a genomic deletion of about 2000 bps. (C) Real-time PCR for C1orf132 long transcript expression on four different MCF12A edited cell colonies in comparison to the unedited cells is shown.