Table 2.
Information about the evaluated reference control genes and neuronal marker genes. The primer pair ID was compatible with the KiCqStart Primers (Sigma-Aldrich). Nomenclature according to HUGO Gene Nomenclature Committee.
| Markers | Gene Name | Primer Pair ID | Forward Primer | Reverse Primer | |
|---|---|---|---|---|---|
| RPS17 | endogenous control | Ribosomal Protein S17 | H_RPS17_1 | CCATTATCCCCAGCAAAAAG | GAGACCTCAGGAACATAATTG |
| TH | dopaminergic neurons | Tyrosine Hydroxylase | H_TH_1 | CAAAATCCACCATCTAGAGAC | CTGACACTTTTCTTGGGAAC |
| CHAT | cholinergic neurons | Choline O-Acetyltransferase | H_CHAT_1 | TCATTTCTTTGTCTTGGATG | TGGAAGCCATTTTGACTATC |
| TPH1 | serotoninergic neurons | Tryptophan Hydroxylase 1 | H_TPH1_1 | AAAGAGCGTACAGGTTTTTC | GTCTCACATATTGAGTGCAG |
| SYP | synaptogenesis marker | Synaptophysin | H_SYP_1 | CCCTTCGGTATTGTTCAAAG | TTTGACTAGGTGGTTAAGGAG |