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FRMD5 expression at transcript (A; RT-qPCR) and protein level (B; Western blot) in TPC1 and BCPAP cell lines transfected with FRMD5-specific siRNA (siFRMD5) and a scrambled control (siNEG). (C) Native yield of FRMD5 in non-transfected TPC1 and BCPAP cells determined by Western blot. β-actin served as a loading control. Graphical data are presented as mean ± SD. *** p < 0.0001.
