Table 3.
Primary antibodies used in the study for Western blotting.
| Antigen | Catalog No. | Type/ Clone (Symbol) |
Dilution/ Blocking Agent |
Source |
|---|---|---|---|---|
| β-actin | 3700 | Mouse monoclonal (IgG2b)/ 8H10D10 |
1:5000/ 5% skimmed milk |
Cell Signaling Technology, Inc. (Beverly, MA, USA) |
| pERK1/2 (T202/Y204) | 4370 | Rabbit monoclonal/ D13.14.4E |
1:2000/ 5% BSA |
Cell Signaling Technology, Inc. |
| tERK1/2 | 9102 | Rabbit polyclonal | 1:2000/ 5% BSA |
Cell Signaling Technology, Inc. |
| P-gp | sc-13131 | Mouse monoclonal (IgG2b)/ G-1 |
1:200/ 5% skimmed milk |
Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA) |
| pPRAS40 (T246) | 13175 | Rabbit monoclonal/ D4D2 |
1:2000/ 5% BSA |
Cell Signaling Technology, Inc. |
| tPRAS40 | sc-517549 | Mouse monoclonal (IgG1)/ 73P21 |
1:200/ 5% skimmed milk |
Santa Cruz Biotechnology, Inc. |
| pSRC (Y416) | 2101 | Rabbit polyclonal | 1:1000/ 5% BSA |
Cell Signaling Technology, Inc. |
| tSRC | 2110 | Mouse monoclonal (IgG1)/ L4A1 |
1:1000/ 5% skimmed milk |
Cell Signaling Technology, Inc. |
| pSTAT3 (Y705) | 4113 | Mouse monoclonal (IgG1)/ M9C6 |
1:2000/ 5% skimmed milk |
Cell Signaling Technology, Inc. |
| pSTAT3 (S727) | 94994 | Rabbit monoclonal/ D8C2Z |
1:1000/ 5% BSA |
Cell Signaling Technology, Inc. |
| tSTAT3 | sc-8019 | Mouse monoclonal (IgG1)/ F-2 |
1:200/ 5% skimmed milk |
Santa Cruz Biotechnology, Inc. |
BSA, bovine serum albumin; p, phosphorylated protein; t, total protein.