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. 2021 Jun 25;22(13):6839. doi: 10.3390/ijms22136839

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Immunohistochemical staining of rat cerebellum by calbindin. (A) negative control group showing high reaction in calbindin in many Purkinje cells (arrow). (B) vehicle group. (C) D-gal group revealing no calbindin reaction in necrotic Purkinje cells (arrowhead). (D) D-gal+TQ group. (E) D-gal+Cur group. (F) D-gal+TQ+Cur group. (G) Quantification of positive Purkinje cells in different groups. Scale bar = 50 µm. Data were analyzed with one-way ANOVA, followed by Tukey’s multiple comparison test. *** p < 0.001 vs. control. ⁺⁺⁺ p < 0.001 vs. vehicle. ^xxx p < 0.001 vs. D-gal. ^ɸ p < 0.05 vs. D-gal+TQ. ^### p < 0.001 vs. D-gal+TQ+Cur. Error bars represent mean ± SD. n = 10.