Figure 6.

RT-induced cellular stress is reduced upon combined metformin treatment in epithelial cells established from normal lung epithelium. BEAS-2B (A,B) and HSAEC1-KT (C,D) lung epithelial cells were analyzed concerning reactive oxygen species (ROS) production (A,C) at 24 h of post RT and metformin (Met) treatment using dihydroethidium staining in combination with flow cytometry analyses. Data are shown as mean values ±SEM of 5–7 independent experiments. p-value indicates: **** p ≤ 0.0001 by two-way ANOVA with post hoc Tukey’s multiple comparison test and additionally by unpaired (two-tailed) t-tests depicted as # p ≤ 0.05, ## p ≤ 0.01. Respective cell cycle analysis (B,D) at that time point were estimated following Nicoletti staining. Data are summarized as mean values ±SEM of 3–5 independent experiments.