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. 2021 Jun 29;22(13):7036. doi: 10.3390/ijms22137036

Figure 1.

Figure 1

SCP-1 cell viability is affected by 16 Hz ELF-PEMFs (0, 7, 30, and 90 min) and 5% cigarette smoke extract (CSE). (A) Fluorescent Calcein-AM staining (2 µM, green) was used to visualize living cells, and Hoechst 33,342 (2 μg/mL, blue) was used as nuclear counterstain. (B) Sulforhodamine B (SRB) staining was used to quantify cell numbers by total protein content after 3 days. N = 3, n = 3. Data are presented as a box plot (Min to Max with single data points). Data were compared by non-parametric two-way ANOVA followed by Tukey’s multiple comparison test: *** p < 0.001 as indicated; # p < 0.05 ### p < 0.001 as compared to the respective control (no ELF-PEMF).