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. 2021 Jun 22;22(13):6660. doi: 10.3390/ijms22136660

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effect of high glucose treatment on the expression of TFs associated with intestinal cell differentiation. Expression of Atoh1 (A), Hes1 (B), Pou2f3 (C), Klf4 (D), Sox9 (E), Neurog3 (F), Neurod1 (G), Foxa1 (H), and Foxa2 (I) in intestinal organoids cultured with high glucose (35 mM) for 48 h. Data generated from three biological replicates from five mice. Unpaired Student t-test: * p < 0.05, ** p < 0.01, *** p < 0.001; n.s. not significant. C = Control (17.5 mM glucose). HG = High Glucose (35 mM glucose).