Table 1.
Primer sets for qPCR analysis.
| Primer Name | NCBI Reference Sequence | Primer Sequence (5’->3’) |
|---|---|---|
| β-actin | EF156276.1 | F: ACTATCGGCAATGAGCGGTTCC R: AGCACTGTGTTGGCATAGAGGTC |
| Type II collagen | NM_012929.1 | F: CGAACCCAAAGGACCCAAAT R: TCCGGACTGTGAGGTTAGG |
| Aggrecan | NM_022190.1 | F: CGAGTGAACAGCATCTACC R: GAGTCATTGGAGCGAAGG |
| iNOS (NOS2) | NM_012611.3 | F: GCTACACTTCCAACGCAACA R: ATGGTGAACACGTTCTTGGC |
| BIM | NM_171988.2 | F: GCAAACGATTACCGAGAGGC R: TCCAGACCAGACGGAAGATG |
| PUMA | NM_173837.2 | F: AAGAGCAACATCGACACCGA R: TCCAGGATCCCTGGGTAAGG |
| NOXA | NM_001008385.1 | F: GAGTGCACCGGACATAACTG R: GCTTGGGCTTCTTCTCATCG |
| Bcl-2 | NM_016993.1 | F: CTGGGGATGACTTCTCTCGT R: GGACATCTCTGCAAAGTCGC |
| BAK | NM_053812.1 | F: CACCCATGCTCCTGTTATGC R: CCCACCCTTGCTACATTTGG |
| BAX | NM_017059.2 | F: TTGGCGATGAACTGGACAAC R: GTAGAAAAGGGCAACCACCC |
iNOS (NOS2): inducible nitric oxide synthase 2; BIM: Bcl-2-like protein 11; PUMA: p53 upregulated modulator of apoptosis; NOXA: phorbol-12-myristate-13-acetate-induced protein 1; Bcl-2: B cell lymphoma 2, apoptosis regulator); BAK: Bcl-2 antagonist/killer 1; BAX: Bcl-2 associated X, apoptosis regulator; F: Forward primer; R: Reverse primer.