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. 2021 Jul 8;9(7):e002267. doi: 10.1136/jitc-2020-002267

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© Author(s) (or their employer(s)) 2021. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See http://creativecommons.org/licenses/by-nc/4.0/.

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Screening of cytokines and growth factors secreted from exPBNK regulated by OS cells with/without dinutuximab +N-803. Purified exPBNK cells were cultured in medium with U2OS (OS) tumor cells with/without IgG, dinutuximab, N-803, or dinutuximab+N-803 for 3 days. OS tumor cells cultured in medium were used as controls. The supernatants were collected after 3 days culture and used for Bio-Plex pro human cytokines screening panel 48 cytokines assay. (A) The combination of exPBNK+N-803+ dinutuximab significantly reduced the secretion of TRAIL, PDGF-BB, and SCGF-β from OS tumor cells (p<0.001). (B) OS significantly inhibited the secretion of RATNES and SDF-1α from exPBNK cells (p<0.001). (C) OS significantly enhanced MIG and IP-10 secreted from exPBNK cells (p<0.001). (D) The combination of OS, N-803 and dinutuximab significantly enhanced the secretion of MIP-1beta from exPBNK cells (p<0.001). (E) OS alone, N-803+ dinutuximab, or OS+N-803+dinutuximab significantly enhanced the secretion of MIP-1alpha from exPBNK cells (p<0.001). NK=exPBNK, Dinu=dinutuximab, OS=U2OS cell line. Data were presented as mean±SEM from three independent experiments. exPBNK, expanded peripheral blood natural killer cell; TRAIL, tumor factor-related apoptosis-inducing ligand; PDGF-BB, platelet-derived growth factor-BB; IP-10, interferon gamma-induced protein 10; MIG, Monokine induced by gamma interferon; MIP, macrophage inflammatory proteins; OS, osteosarcoma; RANTES, regulated uponon activation, normal T cell expressed and presumably secreted; SCGF, stem cell growth factor; SDF, stromal cell-derived factor.