Figure 1.

(A) Characterisation of sEVs derived from cerebrospinal fluid by transmission electron microscopy (TEM). Images show the presence of small spheres smaller than 200 nm, obtained with Invitrogen isolation kit (left, 100 nm bar) and Exiqon isolation kit (right, 200 nm bar). (B) Characterisation of sEV derived from blood plasma by dynamic light scattering (DLS). Graph shows that vesicles isolated from plasma displayed a mean diameter ranging between 30 and 120 nm. Intensity profiles showed that the major sEV population was approximately 50–120 nm in diameter (Invitrogen kit) and a second minor population was approximately 20–50 nm (Exiqon miRCURY kit).