Figure 1.

Effect of cN-II silencing on purine and pyrimidine nucleotide content in NCI-H292 cells. (A) monophosphate nucleoside and NADP (NADP⁺ plus NADPH) content; (B) diphosphate nucleoside content; (C) triphosphate nucleoside and total NAD (NAD⁺ plus NADH) content; (D) energy charge; (E) adenylate pool. Nucleotides were separated by HPCE as described in Materials and Methods. Results are the mean ± SD of three independent experiments and are expressed as a percentage of peak area of each compound normalized to the protein content. Adenylate pool is expressed as the sum of the peak area of each adenylated compound. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.