Figure 2.

Effect of cN-II silencing on NCI-H292 cell proliferation and p21 expression. (A) NCI-H292 pScont and pScNII cell growth with or without 10 µM pifithrin-α was monitored at 0, 24, 48, and 72 h measuring the absorbance at 596 nm of cells stained with crystal violet. Data are the mean ± SD of two experiments performed in sextuplicate. (B) The subfigures are representative images of the densitometries shown in the bar graphs for p21. NCI-H292 pScont and pScNII cells were cultured with or without 10 µM pifithrin-α for 48 h and expression of p21 was analyzed as described in Materials and Methods. β-actin was used as loading control. The ratio between the intensity of the p21 and actin bands was calculated and the results are expressed as the mean ± SD of three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.