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. 2021 Jun 29;22(13):7004. doi: 10.3390/ijms22137004

Figure 4.

Figure 4

Effect of cN-II silencing on enzyme activities, thiols and lactate levels in NCI-H292 cells. (A) Thiols level; (B) hexokinase (HK); (C) citrate synthase (CS) and (D) lactate levels were assayed as described in Materials and Methods. In (A–C), cell extracts were obtained from pScont and pScNII cells incubated for 48 h with or without 10 µM pifithrin-α. In (D) (+) stands for “in the presence of 10 µM pifithrin-α“. Data are the mean ± SD of three independent experiments performed in triplicate for de determination of thiol and enzyme activities and in sextuplicate for lactate. * p < 0.05, ** p < 0.01, *** p < 0.001.