Figure 6.

Effects of sympathetic neuronal depletion in the effector phase of POI. (A–C) Representative FACS images showing the gating strategy of the main population, singlets, and living CD45⁺ cells (A), the ratio of Ly6C⁺ Ly6G^- monocytes and Ly6C⁺ Ly6G⁺ neutrophils (B), monocyte-derived F4/80⁺ macrophages (C), pre-gated on Hoechst^- CD45⁺ of the vehicle- and cSTX-treated mice 24 h after IM. (D–G) Bar graphs showing the absolute numbers of total Hoechst^- CD45⁺ leukocytes (D), Ly6C⁺ Ly6G^- monocytes (E), Ly6C⁺ Ly6G⁺ neutrophils (F), and monocyte-derived F4/80⁺ macrophages (G) isolated from small bowel muscularis externa of vehicle- and cSTX-treated mice 24 h after IM (n = 9). The graphs are plotted as mean ± SEM, and statistical analysis was carried out by two-way ANOVA (* p < 0.05, ** p < 0.01).