Table 1.
Selected DMRM transitions and instrument settings.
| Analyte | Precursor ion (m/z) | Product ion (m/z) | FV (V) | CE (eV) | RT (min) | ΔRT | Polarity |
|---|---|---|---|---|---|---|---|
| QA | 168.0 | 150.0 | 60 | 8 | 1.10 | 1.50 | ESI+ |
| 124.0 | 60 | 8 | |||||
| 3HKyn | 225.0 | 208.0 | 80 | 8 | 1.72 | 1.50 | ESI+ |
| 162.0 | 80 | 10 | |||||
| 3HAA * | 154.0 | 136.0 | 100 | 8 | 1.20 | 1.50 | ESI+ |
| 103.0 | 100 | 20 | |||||
| AA * | 138.0 | 120.0 | 100 | 4 | 2.32 | 1.50 | ESI+ |
| 91.5 | 100 | 16 | |||||
| NAm | 123.0 | 96.0 | 110 | 20 | 2.58 | 2.00 | ESI+ |
| 106.0 | 110 | 20 | |||||
| Kyn | 209.0 | 192.0 | 100 | 8 | 2.64 | 2.00 | ESI+ |
| 174.0 | 100 | 10 | |||||
| 3NT ** | 227.0 | 181.0 | 80 | 8 | 2.80 | 2.00 | ESI+ |
| 117.0 | 80 | 20 | |||||
| XA | 206.0 | 160.0 | 110 | 20 | 3.03 | 2.00 | ESI+ |
| 188.0 | 110 | 8 | |||||
| Kyna | 190.0 | 144.0 | 100 | 20 | 3.32 | 2.00 | ESI+ |
| 172.0 | 100 | 10 | |||||
| Trp | 205.0 | 188.0 | 110 | 8 | 3.52 | 2.00 | ESI+ |
| 159.0 | 110 | 10 |
* Determined only in samples of peritoneal fluid. ** Internal standard. QA: quinolinic acid; 3HAA: 3-hydroxyanthranilic acid; AA: anthranilic acid; 3HKyn: 3-hydroxykynurenine; NAm: nicotinamide; Kyn: kynurenine; Kyna: kynurenic acid; Trp: tryptophan; XA: xanthurenic acid; 3NT: 3-nitrotyrosine; FV: fragmentor voltage; CV: collision energy; RT: retention time.