Table 4.
Recovery, matrix effects, and stability.
| Serum | Peritoneal Fluid | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Analyte | Spiked (µmol/L) | RE ± SD (%) | ME ± SD (%) | Stability ** ± SD (%) | FT Stability ± SD (%) | Spiked (µmol/L) | RE± SD (%) | ME ± SD (%) | Stability ** ± SD (%) | FT Stability ± SD (%) |
| QA | 11.96 * | 83.75 ± 2.44 | 129.56 ± 5.64 | 95.45 ± 9.78 | 84.16 ± 3.68 | 17.94 * | 82.97 ± 3.76 | 73.90 ± 3.96 | 96.02 ± 12.45 | 97.99 ± 9.91 |
| 35.88 * | 95.16 ± 3.14 | 104.40 ± 6.46 | 92.75 ± 12.26 | 93.00 ± 7.13 | 53.82 * | 87.94 ± 1.66 | 86.12 ± 4.23 | 92.40 ± 8.55 | 101.09 ± 9.15 | |
| 1.50 | 86.50 ± 3.36 | 97.39 ± 6.94 | 98.41 ± 1.03 | 121.28 ± 1.27 | 5.98 | 89.58 ± 2.52 | 84.23 ± 7.67 | 96.14 ± 7.86 | 99.01 ± 10.52 | |
| 2.99 | 94.86 ± 1.40 | 109.93 ± 1.36 | 102.33 ± 1.12 | 105.38 ± 1.50 | 11.96 | 90.06 ± 4.02 | 83.74 ± 8.22 | 98.71 ± 8.39 | 100.16 ± 9.37 | |
| 3HKyn | 23.00 * | 92.90 ± 7.35 | 80.92 ± 10.84 | 76.28 ± 12.62 | 79.62 ± 5.28 | 23.00 * | 49.39 ± 5.29 | 89.90 ± 2.86 | 87.87 ± 6.64 | 70.60 ± 9.90 |
| 111.50 * | 88.25 ± 8.00 | 109.19 ± 11.57 | 53.74 ± 10.14 | 73.73 ± 5.16 | 0.07 | 50.40 ± 2.76 | 81.81 ± 2.28 | 87.42 ± 6.69 | 69.10 ± 8.74 | |
| 1.12 | 87.95 ± 7.31 | 87.15 ± 7.04 | 65.51 ± 7.14 | 70.90 ± 12.56 | 2.30 | 50.99 ± 2.06 | 76.59 ± 3.59 | 83.35 ± 4.25 | 64.28 ± 4.95 | |
| 2.23 | 100.35 ± 6.45 | 94.17 ± 8.72 | 64.22 ± 6.92 | 76.46 ± 13.13 | 9.20 | 57.89 ± 4.41 | 77.43 ± 8.77 | 83.70 ± 8.71 | 64.87 ± 6.41 | |
| 3HAA | n.s. | n.s. | n.s. | n.s. | 32.65 * | 91.84 ± 2.74 | 80.40 ± 8.95 | 101.12 ± 13.82 | 94.02 ± 6.12 | |
| n.s. | n.s. | n.s. | n.s. | 97.95 * | 97.18 ± 9.22 | 85.88 ± 7.10 | 101.15 ± 7.27 | 95.61 ± 3.21 | ||
| n.s. | n.s. | n.s. | n.s. | 3.27 | 108.89 ± 4.89 | 85.10 ± 3.54 | 67.71 ± 7.28 | 90.39 ± 8.15 | ||
| n.s. | n.s. | n.s. | n.s. | 13.06 | 112.70 ± 3.63 | 88.10 ± 2.78 | 66.82 ± 5.35 | 93.15 ± 4.99 | ||
| AA | n.s. | n.s. | n.s. | n.s. | 36.46 * | 95.66 ± 9.13 | 77.37 ± 6.44 | 97.89 ± 9.93 | 94.42 ± 3.99 | |
| n.s. | n.s. | n.s. | n.s. | 109.40 * | 96.89 ± 5.37 | 80.58 ± 2.77 | 104.84 ± 7.46 | 105.06 ± 5.63 | ||
| n.s. | n.s. | n.s. | n.s. | 3.65 | 89.98 ± 3.40 | 80.80 ± 9.16 | 92.88 ± 2.18 | 100.43 ± 12.91 | ||
| n.s. | n.s. | n.s. | n.s. | 14.58 | 90.42 ± 2.02 | 87.32 ± 4.10 | 88.95 ± 2.76 | 97.18 ± 4.49 | ||
| NAm | 8.19 * | 91.92 ± 8.56 | 113.53 ± 7.41 | 105.99 ± 9.19 | 76.83 ± 12.19 | 8.19 * | 83.64 ± 7.05 | 126.55 ± 6.53 | 140.51 ± 15.84 | 100.07 ± 7.40 |
| 24.60 * | 93.73 ± 5.63 | 104.40 ± 6.46 | 107.76 ± 9.21 | 108.47 ± 7.48 | 24.60 * | 85.47 ± 6.10 | 114.41 ± 9.97 | 128.61 ± 16.89 | 97.60 ± 6.57 | |
| 2.05 | 97.61 ± 3.52 | 121.20 ± 3.49 | 102.18 ± 1.58 | 98.89 ± 2.51 | 4.10 | 85.89 ± 3.46 | 128.01 ± 1.75 | 104.55 ± 6.60 | 103.33 ± 5.93 | |
| 4.10 | 96.98 ± 3.89 | 154.05 ± 10.24 | 102.56 ± 1.90 | 99.62 ± 0.86 | 16.38 | 85.45 ± 2.08 | 107.11 ± 6.94 | 107.93 ± 4.79 | 104.57 ± 9.49 | |
| Kyn | 4.80 * | 93.61 ± 5.75 | 100.75 ± 3.88 | 105.73 ± 1.85 | 90.93 ± 3.35 | 4.80 * | 85.53 ± 3.33 | 123.75 ± 8.17 | 112.48 ± 10.58 | 98.15 ± 9.62 |
| 14.40 * | 103.62 ± 7.72 | 109.87 ± 6.52 | 108.02 ± 11.62 | 107.62 ± 2.91 | 14.40 * | 86.33 ± 6.10 | 122.74 ± 9.64 | 109.01 ± 12.78 | 98.01 ± 3.71 | |
| 1.20 | 91.11 ± 3.65 | 125.61 ± 4.40 | 110.11 ± 3.18 | 108.58 ± 0.52 | 2.40 | 88.78 ± 1.27 | 121.38 ± 11.30 | 91.92 ± 4.47 | 105.19 ± 6.35 | |
| 2.40 | 96.40 ± 1.75 | 133.20 ± 9.63 | 102.17 ± 0.79 | 109.16 ± 0.84 | 9.60 | 94.43 ± 3.38 | 122.84 ± 10.86 | 97.62 ± 6.84 | 101.01 ± 5.10 | |
| XA | 9.80 * | 81.44 ± 3.58 | 84.55 ± 7.12 | 124.49 ± 7.22 | 126.20 ± 7.64 | 14.25 * | 103.43 ± 8.60 | 113.55 ± 5.38 | 92.28 ± 6.76 | 83.76 ± 6.29 |
| 29.40 * | 87.09 ± 7.31 | 72.26 ± 2.98 | 90.99 ± 6.38 | 93.19 ± 5.58 | 42.75 * | 109.06 ± 8.95 | 115.42 ± 1.22 | 103.87 ± 4.20 | 90.31 ± 8.12 | |
| 0.74 | 85.17 ± 4.16 | 78.61 ± 8.25 | 103.26 ± 1.06 | 99.01 ± 5.82 | 2.38 | 110.33 ± 8.66 | 97.24 ± 4.25 | 82.58 ± 5.39 | 87.07 ± 7.58 | |
| 1.47 | 86.13 ± 3.62 | 79.80 ± 4.61 | 99.10 ± 5.12 | 99.63 ± 1.71 | 9.50 | 111.00 ± 4.33 | 100.46 ± 1.84 | 84.56 ± 5.26 | 84.43 ± 8.02 | |
| Kyna | 5.30 * | 91.82 ± 2.73 | 83.76 ± 1.44 | 98.64 ± 2.46 | 111.62 ± 4.28 | 5.32 * | 87.88 ± 5.18 | 119.91 ± 10.49 | 140.90 ± 14.55 | 92.32 ± 6.14 |
| 31.80 * | 98.41 ± 6.58 | 95.35 ± 2.27 | 107.32 ± 6.25 | 109.01 ± 7.80 | 15.96 * | 91.94 ± 7.02 | 113.44 ± 11.32 | 131.72 ± 14.84 | 97.08 ± 3.90 | |
| 1.33 | 99.59 ± 2.79 | 91.74 ± 7.54 | 103.74 ± 1.88 | 107.67 ± 0.79 | 2.66 | 108.88 ± 4.60 | 105.65 ± 0.98 | 98.89 ± 3.61 | 103.82 ± 4.67 | |
| 2.65 | 101.85 ± 1.32 | 90.71 ± 0.34 | 102.10 ± 1.12 | 106.99 ± 0.30 | 10.64 | 113.62 ± 3.33 | 103.96 ± 1.97 | 92.33 ± 3.58 | 96.53 ± 1.58 | |
| Trp | 2.45 * | 88.27 ± 2.92 | 86.76 ± 2.61 | 92.76 ± 4.05 | 97.55 ± 3.02 | 2.45 * | 95.29 ± 6.37 | 114.70 ± 11.78 | 123.18 ± 6.03 | 98.17 ± 3.72 |
| 7.35 * | 88.10 ± 7.12 | 66.07 ± 6.20 | 88.21 ± 10.57 | 89.20 ± 3.45 | 7.35 * | 94.51 ± 2.85 | 97.32 ± 11.01 | 118.11 ± 5.61 | 98.86 ± 6.53 | |
| 1.23 | 88.96 ± 2.61 | 71.47 ± 4.16 | 87.70 ± 3.24 | 99.79 ± 2.31 | 2.45 | 99.12 ± 1.46 | 93.09 ± 3.63 | 96.63 ± 8.19 | 104.80 ± 6.49 | |
| 2.45 | 87.51 ± 2.48 | 74.50 ± 1.15 | 89.94 ± 3.18 | 99.44 ± 0.15 | 9.80 | 115.35 ± 3.83 | 101.14 ± 3.95 | 104.82 ± 4.51 | 109.17 ± 9.42 | |
| 3NT | 2.21 | 98.23 ± 2.55 | 101.82 ± 2.27 | 101.15 ± 5.33 | 98.20 ± 1.70 | 55.25 | 104.97 ± 2.51 | 97.19 ± 4.85 | 81.14 ± 4.75 | 89.54 ± 4.01 |
* Values in nmol/L. ** Stability after keeping samples in an autosampler for 24 h. SD: standards deviation (n = 6); n.s.: not studied; RE: recovery; ME: matrix effect; FT Stability: freeze–thaw stability.