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. 2021 Jun 28;22(13):6952. doi: 10.3390/ijms22136952

Figure 8.

Figure 8

PwNAC11 and ABF3 cooperatively activates ERD1 transcription. (A) Diagram of the ERD1 promoter. The seven ABREs detected in ERD1 promoter are indicated in green and one DRE is marked in yellow. (B) Yeast one-hybrid assays for the interaction of PwNAC11 with ERD1 promoter, ABRE and DRE element. Aureobasidin A (AbA) of 500ng/mL was used to inhibit autoactivation. pGADT7-p53 and pAbAi-p53 were used as positive control. Empty PGADT7 was used as negative control. (C) Sketch map of the double-effector and reporter plasmids in dual-luciferase reporter assay. (D) Dual-luciferase assays for PwNAC11 and ABF3 interactions. (E) Dual-luciferase assays for PwNAC11 and DREB2A. The data are represented as means ± standard error from three independent replications. The grey lines indicate the comparisons and asterisks indicate significant differences compared with SK (* p < 0.05; ** p < 0.01; *** p < 0.001).