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. 2021 Jun 23;22(13):6746. doi: 10.3390/ijms22136746

Figure 8.

Figure 8

Immunofluorescence (IF) analysis of organotypic skin cultures using epidermis-specific markers: keratin 5 (K5), characteristic of basal epidermal cells (red staining, in (A,B)); keratin 10 (K10), characteristic of suprabasal epidermal cells (green staining, in (A,B)); filaggrin, characteristic of the stratum granulosum, the last compartment containing living cells before dead and cornified cells (green staining, in (A,B)) and collagen IV (Col IV), component of the lamina densa of basememnt membrane (green staining in (C)). (A) Organotypic skin cultures (1.2 mg/mL); (B) organotypic skin cultures (2.4 mg/mL) after 15 days of differentiation at the air-liquid interface; (C) Organotypic skin culture (2.4 mg/mL) after 25 days of differentiation at the air-liquid interface. SC: Stratum Corneum; E: epidermis; D: dermis; BM: Basement Membrane. Dotted white line indicates the dermo-epidermal junction (basal membrane). Scale bar: 500 µm.