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. 2021 Jun 25;22(13):6842. doi: 10.3390/ijms22136842

Figure 4.

Figure 4

PCu attenuates Cu2+-mediated cell damage and oxidative stress. (A) N2a-sw cells incubated with different concentrations of Cu(II) or 100 μM PCu for 12 h to detect cytotoxicity in N2a-sw cells using MTT assay. (B) Release of LDH (C) The production of ROS. (D) SOD activity in N2a-sw cells. (E) Representative photomicrographs of JC-1 fluorescent staining in the N2a-sw cells. Scale bar: 10 μm. The data represent the mean ± S.E. of three independent experiments. * p < 0.05; ** p < 0.01; *** p < 0.001 compared to the Cu2+-damaged group.