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. 2021 Jun 24;12:669162. doi: 10.3389/fimmu.2021.669162

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Copyright © 2021 Saisorn, Saithong, Phuengmaung, Udompornpitak, Bhunyakarnjanarat, Visitchanakun, Chareonsappakit, Pisitkun, Chiewchengchol and Leelahavanichkul

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Renal ischemia reperfusion injury (I/R) at 24 h induced more prominent neutrophil extracellular traps (NETs) in blood of lupus prone mice. Characteristics of NETs in peripheral blood neutrophils from Fcgr2b-/- or wild-type (Fcgr2b+/+) mice after renal ischemia reperfusion injury (I/R) as determined by the nuclear morphology by 4’,6-diamidino-2-phenylindole (DAPI) staining (blue color) (A), co-staining of myeloperoxidases (MPO) and neutrophil elastase (NE) (B), citrullinated histone H3 (CitH3) staining (C) with the representative pictures from peripheral blood neutrophils at 24 h post-I/R (or sham) from both mouse strains with DAPI staining (original magnification 200x) (D), and immunofluorescence of MPO/NE and CitH3 (original magnification 630x) (E, F) are demonstrated. Additionally, the gene expression of PAD4 and IL-1β (G, H) together with serum IL-1β (I) and serum ds-DNA (J) are also demonstrated (n = 6–7/time-point).