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. 2021 Jun 24;12:669162. doi: 10.3389/fimmu.2021.669162

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Copyright © 2021 Saisorn, Saithong, Phuengmaung, Udompornpitak, Bhunyakarnjanarat, Visitchanakun, Chareonsappakit, Pisitkun, Chiewchengchol and Leelahavanichkul

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Renal ischemia reperfusion injury (I/R) at 24 h induced more prominent neutrophil extracellular traps (NETs) in renal glomeruli of lupus prone mice. Characteristics of NETs in kidneys from Fcgr2b-/- or wild-type (Fcgr2b+/+) mice after renal ischemia reperfusion injury (I/R) as determined using co-detection of myeloperoxidases (MPO) and neutrophil elastase (NE) at 24 h post-I/R (A) (n = 5–8/group) and gene expression in the time-point of IL-1β and PAD4 (B, C) (n = 6–7/time-point) with the representative immunofluorescent pictures of NE (green), MPO (red) and 4’,6-diamidino-2-phenylindole (DAPI, blue nuclear staining) at 24 h post-I/R (or sham) (original magnification 630x) (D) are demonstrated.