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. 2021 Jun 24;12:669162. doi: 10.3389/fimmu.2021.669162

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Copyright © 2021 Saisorn, Saithong, Phuengmaung, Udompornpitak, Bhunyakarnjanarat, Visitchanakun, Chareonsappakit, Pisitkun, Chiewchengchol and Leelahavanichkul

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Renal ischemia reperfusion injury (I/R) at 24 h induced more prominent neutrophil extracellular traps (NETs) in renal glomeruli of lupus prone mice. Characteristics of NETs in kidneys from Fcgr2b-/- mice or wild-type (Fcgr2b+/+) mice after renal ischemia reperfusion injury (I/R) as determined by immunofluorescent staining of citrullinated histone H3 (CitH3) at 24 h post-I/R (A) (n = 5–6/group) and IL-1β from renal tissue (B) (n = 5–6/time-point) with the representative immunofluorescent pictures of CitH3 (red) and 4’,6-diamidino-2-phenylindole (DAPI, blue nuclear staining) at 24 h post-I/R (or sham) (original magnification 630x) (C) are demonstrated.