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. 2021 Jun 24;12:669162. doi: 10.3389/fimmu.2021.669162

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Copyright © 2021 Saisorn, Saithong, Phuengmaung, Udompornpitak, Bhunyakarnjanarat, Visitchanakun, Chareonsappakit, Pisitkun, Chiewchengchol and Leelahavanichkul

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Prominent neutrophil extracellular traps (NETs) at 4 h of the stimulation in neutrophils of lupus prone mice. The representative immunofluorescent pictures of NETs formation in Fcgr2b-/- and wild-type (Fcgr2b+/+) neutrophils after 4 h activation by phorbol myristate acetate (PMA), a NETs activator, or lipopolysaccharide (LPS), a TLR-4 stimulator, as evaluated by the co-staining of myeloperoxidases (MPO) and neutrophil elastase (NE) (upper part) or citrullinated histone H3 (CitH3) (lower part) are demonstrated.