Figure 3.

IL-6 and TNF-α quantification by ELISA: The concentration of IL-6 (A) and TNF-α (B) was measured after 7, 14, and 21 days in supernatants of ADSCs cultured in the presence of adipogenic differentiation medium (MD) (blue bars), or in MD plus vitamin D (MD+VIT) (yellow bars), or in MD plus metformin (MD+MET) (orange bars), or in MD with both metformin and vitamin D (MD+VIT+MET) (red bars), as compared to control untreated cells (grey bars). Data are expressed as mean ± SD referred to the control (mean ± SD; n = 6) (* p ≤ 0.05; ** p ≤ 0.01).