Figure 5.

Immunohistochemistry analysis of FoxO1 after 21 days of differentiation: Immunohistochemical analysis of the expression of FoxO1 was performed in ADSCs cultured in the presence of adipogenic differentiation medium (MD), or in MD plus vitamin D (MD+VIT), or in MD plus metformin (MD+MET), or in MD with both metformin and vitamin D (MD+VIT+MET), as compared to control untreated cells (Ctrl). The figures are representative of two different independent experiments with three technical replicates. For each differentiation marker, fields with the highest yield of positively stained cells are shown. Nuclei are labelled with 4,6-diamidino-2-phenylindole (DAPI, blue). Scale bars: 40 µm.