Figure 3.

Sanger sequencing results of transgenic plants derived from immature embryo transformation. Two gRNAs are underlined. PAM sequences are highlighted in bold. gRNA1: 5′-CCGGCGAGGAGGCTGTCCATCAC-3′; gRNA2: 5′-ACGGCCGCTGCCGGATGCCGTGG-3′. gRNA1 and gRNA2 are 350 bp apart. "WT" indicates that the allele was not edited (wild type).