Figure 1.
Photocurrents elicited with different intensities and durations. mVChR1 expression was observed as Venus fluorescence (green), while the plasma membrane and nuclei were stained with WGA (red) and DAPI (blue), respectively (A). mVChR1 expression was clearly localized in the plasma membrane. Photocurrents were recorded by stimuli with 560 nm LEDs at various intensities and durations ((B), n = 8). Typical waveforms were recorded with a stimulus at 0.1, 1.0, and 10 µW/mm2 for 10 ms (C). mVChR1, modified volvox-derived channelrhodopsin-1.