Figure 3.

Comparison of the number of RGCs after continuous light exposure. Retrograde labeling of RGCs (blue), mVChR1-expressing cells (green), and merged photographs are shown in (A) (no light exposure) and (B) (continuous light exposure). The number of total and mVChR1-positive RGCs was counted (C). The RGCs were counted by dividing them into superior and inferior parts (D). Cell sizes in the retina without light exposure and the retina with continuous light exposure were analyzed (E); both cell sizes were well correlated (r² = 0.9871). mVChR1, modified volvox-derived channelrhodopsin-1; RGC, retinal ganglion cells.