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. 2021 Jun 23;22(13):6749. doi: 10.3390/ijms22136749

Figure 3.

Figure 3

The effect of external AtCHS- and NPTII-encoding dsRNAs on AtCHS mRNA level and total anthocyanin content in Arabidopsis thaliana. (a) Arabidopsis plants grown under control (+22 °C, 16 h light, upper panel) and anthocyanin-inducing (+7 °C, 23 h light, lower panel) conditions for seven days after treatment with sterile water or synthetic dsRNA. (b) Quantitative real-time PCR measuring relative mRNA levels of endogenous AtCHS in the leaves of A. thaliana treated with water or synthetic dsRNAs. (c) HPLC results of total anthocyanins in the leaves of A. thaliana grown under the control and anthocyanin-inducing conditions. WC—A. thaliana treated with sterile water; CHS—A. thaliana treated with AtCHS-dsRNAs; NPT—A. thaliana treated with NPTII-dsRNA; dpt—days post-treatment. The data are presented as the mean ± SE (three independent experiments). Means followed by the same letter were not different using Student’s t test. p < 0.05 was considered statistically significant.