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. 2021 Jun 23;22(13):6749. doi: 10.3390/ijms22136749

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Detection of Cy3-labeled AtCHS-dsRNA (red) in the externally treated Arabidopsis thaliana leaves by laser scanning microscopy. Adaxial (a–c) and abaxial (d–h) leaf sides of four-week old A. thaliana were exogenously treated with the labeled AtCHS-dsRNA (red) by sterile soft brushes and analyzed 13–15 h post-treatment. (a) Negative control (sterile water); (b–h)—AtCHS-dsRNA treatment. Green color results from the autofluorescence of chloroplasts. Red color results from the Cy3-labeled dsRNA. Scale bars, 100 μm. Three independent trials showed the same results.