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. 2021 Jun 27;22(13):6901. doi: 10.3390/ijms22136901

Figure 5.

Figure 5

Skin confocal immunofluorescence analysis. Staining for p63 (Green) and K1 (Red). (A) p63 and K1 staining merged with DAPI (Blue) (B) K1 single channel. (C) p63 single channel (AC, 20× objective). (D) High magnification capture (60× objective) showing nuclear localization of p63 and K1 filament clumping (stars, please see high magnification capture furnished in Figure S1); scale bars = 50 μm. (E) K1 single channel. (F) p63 single channel. Immunofluorescence analysis for p63 and K1 antigens. P63 is identified using Alexa fluor®488 igG (FITC green fluorescence), laser at 488 nm and 525/50 band pass filter while K1 with Alexa fluor®568 igG (TRITC red fluorescence), laser at 561 nm and 595/50 band pass filter. Nuclei detection was performed using DAPI (Blu fluorescence), laser at 404 nm and 450/50 band pass filter.

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