FIG 1.

Bacterial high-content imaging. (A) Schematic of the bacterial high-content imaging workflow. Overnight bacterial cultures are added to ultrathin bottom plates and incubated with or without antimicrobial compounds. Adherent bacteria are fixed and stained before being imaged on an Opera Phenix high-content confocal microscope using a 63× water immersion objective. Images were analyzed using Harmony software, and data were exported and plotted in R. (B) Representative image of K. pneumoniae NCTC 43816 stained with FM4-64 (cell membrane), DAPI (nucleic acid, membrane permeative), and SYTOX green (nucleic acid, membrane impermeative).