TABLE 1.

Nucleotide and amino acid differences between VOCs and ancestral isolates^a

Reaction	Genome position		nt				aa
	nt	aa	WT	B.1.1.7	B.1.351	P.1	WT	B.1.1.7	B.1.351	P.1
nPCR C	21765–21770	69–70	ACATGT	del	ACATGT	ACATGT	HV	del	HV	HV
	21801	80	A	A	C	A	D	D	A	D
	21974	138	G	G	G	T	D	D	D	Y
	21991–21993	144	TTA	del	TTA	TTA	Y	del	Y	Y
nPCR D	23012	484	G	G	A	A	E	E	K	K
	23063	501	A	T	T	T	N	Y	Y	Y
	23271	570	C	A	C	C	A	D	A	A

^aWT, Wuhan-Hu-1 strain (GenBank accession number MN908947). This strain was first detected in the UK (B.1.1.7), South Africa (B.1.351), or Brazil (P.1). Single-letter amino acid codes are used. Changes from the WT sequence are italicized and underlined. Primers for nPCR reactions are shown in Table S1 in the supplemental material. Abbreviations: nt, nucleotides; aa, amino acids.