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. 2021 May 26;6(3):e01058-20. doi: 10.1128/mSystems.01058-20

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FIG 3 — MetFish is applicable to measuring metabolites with a broad range of functional groups in challenging sample matrices. Shown are extracted ion chromatograms with the transitions obtained in selected reaction monitoring (SRM) mode for metabolite quantification from application of MetFish in measurement of (a) amine metabolites, (b) carboxyl metabolites, (c) carbonyl metabolites, (d) hydroxyl metabolites as sugars, and (e) hydroxyl metabolites as alcohols. In all cases, MetFish was deployed in situ in metabolite-salt mixtures containing 2 M MgSO₄.