FIG 4.

Application of MetFish in quantification of proteinogenic amino acids in representative microbial communities. (a) Quantification of amino acids during phototrophic microbial community succession with various nitrogen amendments (data shown are normalized average amino acid concentrations from analysis of 3 biological replicate succession experiments). (b) Exo- and endometabolomics analysis of amino acids in soil, using a high-salt wash to increase recovery due to possible disruption of nonspecific binding to soil particles (data shown are mean ± standard deviation from analysis of 3 replicate soil samples).