Figure 2.

Experimental fixation scheme of the two campaigns. (A) The macrophages were separated and integrated into the TEXUS-54 hardware 5.5 h prior to launch. The rocket was launched at 10:30. After 50 s, the first hypergravity (hypg) sample was fixed. Then, 375 s after launch, the microgravity (µg) sample was fixed. Two ground controls that were integrated into the hardware but not launched were taken at the corresponding timepoints of the different fixation times. (B) The CELLBOX-PRIME hardware was uplifted to the ISS. After 11 days in microgravity, cells were fixed and stabilized, thereby stopping biological activity. After another 19 days, samples were recovered and prepared for analysis. Corresponding ground controls that were integrated into the same hardware and that were exposed to the same temperature profile as the flight samples were fixed at the same dates.