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. Author manuscript; available in PMC: 2021 Jul 9.
Published in final edited form as: Dev Biol. 2019 Jul 15;455(1):73–84. doi: 10.1016/j.ydbio.2019.07.011

Fig. 4.

Fig. 4.

Tie1 attenuation leads to AoV reduced stiffness. A,B, Two channel fluorescence images (blue: nuclei and red: αSMA) of leaflet sections for each group overlaid with the AFM height channel. C,D, Stiffness values overlaid on 3D topographical maps that correspond to scanned regions in fluorescent images. E, Modulus distributions of AV leaflet AFM scans from Tie1fl/lz;Nfatc1enCre and Tie1fl/fl mice. Drawn vertical lines represent median modulus values, which are aggregated and plotted in (F). AV leaflets from Tie1fl/fl mice were significantly stiffer (p<0.001) than Tie1fl/lz;Nfatc1enCre valve leaflets.