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The area occupied by colonies formed after 14 days of incubation of WM164 VDR KO was greater than scramble cells. Cells were seeded at 3 × 103/well and after 14 days were fixed and dyed with crystal blue. The images of wells were made using a Cytation 5 reader with Gen 5.3 software, and % area coverage relative to the scramble control (100%) was determined. Statistical analysis was via t-test, p < 0.01 **, n = 6.
