Figure 9.
The effect of vitamin D derivatives on the migration of WM164 scramble cells determined with the scratch assay. Movement of cells into the scratch area was observed over 70 h of incubation with secosteroids at 10−7 M or the ethanol vehicle as control. Pictures of the scratch area were taken every hour for 70 h using a Cytation 5 instrument and were analyzed using Gen 5.3 software (BioTek, Winooski, VT, USA), two-way ANOVA, p < 0.05 *, p < 0.01 **, p < 0.001 ***, p < 0.0001 ****, n = 7. The line defines all time points with the same statistical significance.