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. 2021 Jul 5;22(13):7226. doi: 10.3390/ijms22137226

Figure 4.

Figure 4

Trophoblast cell lines disadvantage upon lentiviral YB-1 downregulation. Representative Western Blot image of YB-1 protein levels 7 days upon YB-1 downregulation (A). Effects of YB-1 downregulation on proliferative rates (B), apoptosis in HTR8/SVneo cells (C), and apoptosis related genes expression (D) in trophoblast cell lines. The results from three independent experiments (except A) were statistically analyzed using one-way ANOVA. Data are presented as mean with SD; * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. Scale bar 50 μm. BAX= BCL2-associated X, BCL2 = B-cell lymphoma 2, CASP3 = Caspase 3, CASP9 = Caspase 9, FOSL1= Fos-related antigen 1, LGALS3= Galectin-3.