Figure 5.

Negative effects of YB-1 on migration and invasion in HTR8/SVneo cells. YB-1 downregulation effects on trophoblast cell line viability (A), migration (B), invasion (C), and expression levels of genes relevant for trophoblast migration, invasion, and overall function (D). The results from three independent experiments were statistically analyzed using one-way ANOVA. Data are presented as mean with SD; * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. Scale bar 50 $\mathsf{\mu }$m. MIF = Macrophage migration inhibitory factor, MMP2 = Metalloproteinase 2, MMP9 = Metalloproteinase 9, TIMP1 = Metallopeptidase inhibitor 1, SERPINE1 = Serpin family e member 1, NOTCH1 = Notch homolog 1, NOTCH3 = Notch homolog 3, STAT3 = Signal transducer and activator of transcription 3, NF-$\kappa$B = Nuclear factor kappa-light-chain-enhancer of activated B cells.