TABLE 1.

DNA Sequences for in vitro Selection ^a

DNA name	DNA sequence (5′−3′)
Lib‐N35‐pool	pGGACAAGAGGGGGATCTTGT‐N35‐GTTGTCAGCAGTCTGTCCAT
Primer 1 (P1)	ATGGACAGACTGCTGACAAC
Primer 2 (P2)	Biotin‐TGCTGACAACTrAGGACAAGAGGGGGA
FAM‐substrate	FAM‐ATGGACAGACTGCTGACAACTrAGGACAAGAGGGGGA

DNAzyme is a functional nucleic acid, a single‐stranded DNA molecule with catalytic activity, that can be isolated from random DNA libraries by selection technology. We successfully screened the DNAzyme (PAE‐1) with high specificity and sensitivity to directly detect P. aeruginosa without secondary elution. The PAE‐1 could detect P. aeruginosa at 1.2 cfu/ml in 10 min using our designed DNAzyme‐based sensor.

^a p: phosphorylation; N35: 35 random nucleotides; The ribose adenine cleavage site is represented by rA; FAM: Carboxyfluorescein.