Table 2.
The expressions of majority 43 out of 52 (82.7%) (non-changed and upregulated) coronavirus replication-transcription complex (RTC)-proximal host factors (PMID: 30632963) in coronavirus-infected cells indicate that endothelial cells and other cells may use these factors for viral replication and RNA transcription,which may be novel therapeutic targets.
| HMECs | Calu-3 cells | Mouse Lung tissues | ||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Gene symbol | Significance | ic-MERS | ic-SARS | ic-SARS deltaORF6 mutant | MA-15 (SARS-CoV) | |||||||||||||
| [with 5 PFU (plaque-forming unit) per cell] | (with 105 PFU per mouse) | (with 105PFU per mouse) | ||||||||||||||||
| 12(hr) | 24(hr) | 36(hr) | 48(hr) | 12(hr) | 24(hr) | 36(hr) | 48(hr) | 72(hr) | 12(hr) | 24(hr) | 36(hr) | 48(hr) | 72(hr) | 2(d) | 4(d) | 7(d) | ||
| Translation | ||||||||||||||||||
| EIF3E | **** | -1.56 | -1.70 | -1.10 | ||||||||||||||
| EIF3F | **** | -1.55 | -1.49 | -2.01 | -2.46 | -1.45 | -1.22 | -1.82 | -1.57 | |||||||||
| EIF3I | **** | -1.18 | -1.91 | -1.83 | -1.21 | -1.20 | ||||||||||||
| RPL13A | **** | -1.25 | -1.18 | -1.47 | -1.48 | -1.09 | -1.12 | |||||||||||
| RSL24D1 | ** | -1.41 | -1.65 | |||||||||||||||
| Transport | ||||||||||||||||||
| AAK1 | * | 1.73 | -1.51 | -1.43 | -1.15 | 1.35 | 1.06 | |||||||||||
| ACBD5 | ** | -1.09 | -2.44 | -1.49 | -1.46 | |||||||||||||
| BTF3 | ** | -2.01 | -2.05 | -1.71 | -1.73 | -1.98 | -1.17 | -1.01 | -1.29 | -1.06 | ||||||||
| CLINT1 | * | 1.00 | -1.44 | -1.46 | -1.18 | |||||||||||||
| DNM1L | * | -1.02 | ||||||||||||||||
| GBF1 | *** | 1.52 | ||||||||||||||||
| KIF11 | *** | -1.19 | 2.06 | -1.03 | -1.28 | -2.04 | 2.30 | 2.25 | ||||||||||
| KPNB1 | ** | -1.00 | -1.09 | |||||||||||||||
| NACA | ** | -1.76 | -1.85 | -1.25 | -1.07 | -1.21 | -1.10 | |||||||||||
| RANGAP1 | * | 1.16 | 1.27 | |||||||||||||||
| SCFD1 | ** | -1.09 | ||||||||||||||||
| SNRPE | **** | -1.46 | -1.21 | -1.92 | -1.77 | -1.27 | -1.34 | |||||||||||
| SNX9 | ** | -1.05 | -1.15 | |||||||||||||||
| SRP54A | *** | |||||||||||||||||
| SRP68 | * | 1.00 | -1.18 | |||||||||||||||
| STX5A | ** | |||||||||||||||||
| TNPO1 | * | |||||||||||||||||
| VAPA | * | 1.23 | -1.02 | -1.44 | -2.14 | -1.42 | 1.09 | 1.33 | ||||||||||
| YKT6 | ** | -1.42 | -1.41 | |||||||||||||||
| ZFYVE1 | * | -1.40 | -1.03 | 1.73 | 1.63 | |||||||||||||
| Catabolic process | ||||||||||||||||||
| DNAJC10 | * | -1.10 | -1.06 | -1.71 | -1.84 | -1.93 | -1.26 | -1.77 | -1.83 | |||||||||
| FAM134B | * | -1.96 | -2.93 | -2.04 | -1.17 | -1.69 | -1.09 | |||||||||||
| PSMB3 | * | -1.24 | -2.10 | -1.82 | -1.57 | -1.53 | -1.02 | |||||||||||
| PSMC2 | ** | -1.38 | -1.09 | |||||||||||||||
| PSMD1 | *** | 1.17 | -1.10 | |||||||||||||||
| TFEB | ** | -4.41 | -4.73 | -4.23 | -3.41 | |||||||||||||
| Cell organization | ||||||||||||||||||
| CKAP5 | -1.34 | -1.40 | ||||||||||||||||
| PXN | * | -1.13 | -1.56 | -1.56 | -1.12 | |||||||||||||
| Others | ||||||||||||||||||
| BAG3 | * | 1.88 | 5.25 | 4.84 | 2.76 | 1.82 | 3.04 | 2.55 | ||||||||||
| BPNT1 | * | -1.49 | 1.86 | -1.62 | -1.07 | -1.67 | -1.43 | -1.53 | ||||||||||
| CHD7 | * | -1.75 | 1.62 | 1.08 | ||||||||||||||
| HAUS6 | * | 1.05 | -1.03 | -1.80 | -1.97 | -1.10 | ||||||||||||
| IRS1 | * | -1.92 | ||||||||||||||||
| ITPRIPL1 | * | 1.31 | -1.74 | -1.32 | ||||||||||||||
| KAT7 | * | -1.58 | -1.60 | |||||||||||||||
| LRRFIP1 | * | -1.14 | 1.02 | -1.26 | -1.68 | -1.09 | -1.04 | -1.31 | ||||||||||
| NUDCD1 | *** | |||||||||||||||||
| PKMYT1 | * | -1.15 | 1.20 | 1.02 | ||||||||||||||
| POLR2B | * | -1.11 | -1.15 | |||||||||||||||
| PRMT7 | * | -1.07 | -1.65 | -1.64 | -1.04 | |||||||||||||
| RRM2 | *** | -1.07 | 1.02 | 1.78 | -1.29 | -1.89 | -2.38 | -1.13 | -1.92 | 2.46 | 2.82 | |||||||
| S100A11 | *** | -1.37 | -2.07 | -1.87 | -1.36 | -1.43 | -1.13 | |||||||||||
| STAT5A | ** | 1.55 | 1.99 | |||||||||||||||
| TBCB | * | -1.06 | -2.09 | -2.23 | -1.27 | -1.45 | -1.84 | -1.59 | ||||||||||
| UHRF1 | * | -1.68 | 1.01 | 2.23 | 2.28 | -1.18 | -1.27 | -1.45 | 1.29 | 2.62 | 2.77 | |||||||
| UTP20 | * | 1.38 | 1.50 | 1.35 | 1.06 | 1.04 | ||||||||||||
| UTP3 | * | 1.03 | ||||||||||||||||
Tfh, follicular helper T cell; Th, T helper cell; Treg, regulatory T cell; TAM, tumor-associated-macrophage; NK, natural killer cell; DC, dendritic cell; None, correlation without adjustment; Purity, correlation adjusted for tumor purity; Cor, R value of Spearman’s correlation. *P < 0.01; **P < 0.001; ***P < 0.0001, ****P < 0.0001.