Table 1. SPPS of MUC1 (Glyco)peptides and Their Characterization by MALDI-TOF MS and RP-HPLC.

			MALDI-TOF MS [M + H]+a		RP-HPLC
entry	amino acid sequenceb	no. of sugars	expected (Da)	observed (Da)	tRc (min)
6	H-HGVTSAPDTRPAPGSTAPPA-NH2	0	1884.93	1885.65	17.42
7	H-HGVT*SAPDTRPAPGSTAPPA-NH2	1	2089.25	2088.73	16.34
8	H-HGVTSAPDT*RPAPGSTAPPA-NH2	1	2089.25	2088.49	16.85
9	H-HGVTSAPDTRPAPGST*APPA-NH2	1	2089.25	2088.44	16.57
10	H-HGVTSAPDT*RPAPGST*APPA-NH2	2	2292.45	2290.19	15.93
11	H-HGVT*SAPDTRPAPGST*APPA-NH2	2	2292.45	2291.47	15.38
12	H-HGVT*SAPDT*RPAPGSTAPPA-NH2	2	2292.45	2290.39	15.62
13	H-HGVT*SAPDT*RPAPGST*APPA-NH2	3	2495.28	2493.69	15.11

^aThe matrix used was α-cyano-4-hydroxycinnamic acid.

^bT* is a Thr O-linked Tn monosaccharide.

^cHPLC analysis conditions can be found in the Figures S10–S25.